Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: Viability of A549 cells after prolonged (24, 48 and 72 h) exposure to PBDEs at concentrations from 1.5 to 48 μg mL −1 determined with the MTS assay; 0 μg mL −1 represents untreated cells. The results are expressed as percentage of corresponding control, untreated cells and given as means ± SD ( n = 6). The dashed frame highlights a time-dependent effect. & p < 0.05; # p < 0.01; * p < 0.0001 vs. untreated control.

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques: MTS Assay, Control

Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: Cytotoxicity curves of BDE-99 and -209 at concentrations from 1.5 to 48 μg mL −1 in A549 cells over 24, 48 and 72 h. The results of the MTS assay are given as means ± SD ( n = 6).

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques: MTS Assay

Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: IC 50 values of BDE-99 and -209 in A549 cells over 24, 48 and 72 h.

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques:

Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: Prolonged exposure of A549 cells to a house dust sample extract. A549 cells were exposed to ∑PBDE in house dust at concentrations from 0.375 to 24 μg mL −1 for 24, 48 and 72 h, and cell viability was determined with the MTS assay. The results are given as means ± SD ( n = 6). The dashed frame highlights the concentration found in the house dust sample. & p < 0.05; # p < 0.01; vs. untreated control.

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques: MTS Assay, Concentration Assay, Control

Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: LOAEL cytotoxicity of BDE-99 (7.5 μg mL −1 ) and BDE-209 (15 μg mL −1 ), alone and combined, in A549 cells treated for 24, 48 and 72 h. The results of the MTS assay are given as means ± SE ( n = 6). # p < 0.01; $ p < 0.001 vs. untreated control.

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques: MTS Assay, Control

Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: Levels of LDH release after 4 h exposure of A549 cells to PBDE congeners and house dust sample extract. Triton (0.08%) was used as the positive control. The results are presented as percentage of LDH release and given as means ± SD ( n = 3). # p < 0.01; $ p < 0.001; * p < 0.0001 vs. untreated control.

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques: Positive Control, Control

Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: ROS levels after the 4 h exposure of A549 cells to PBDE congeners, house dust sample extract and H 2 O 2 (100 µM) as positive control. The results of DCF-fluorescence signal measurements are presented as relative fluorescence units (RFU) and given as means ± SD ( n = 6). & p < 0.05; # p < 0.01; $ p < 0.001; * p < 0.0001 vs. untreated control.

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques: Positive Control, Fluorescence, Control

Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: GSH levels after the 4 h exposure of A549 cells to single PBDEs and house dust sample extract at 3, 6 and 12 µg mL −1 and to tBOOH (100 µM) as positive control. The results of MCB-fluorescence measurements are presented as relative fluorescence units (RFU) and given as means ± SD ( n = 6). & p < 0.05; # p < 0.01; $ p < 0.001; * p < 0.0001 vs. untreated control.

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques: Positive Control, Fluorescence, Control

Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: Mitochondrial membrane potential after 4 h exposure of A549 cells to single BDE congeners and house dust sample extract at 3, 6 and 12 µg mL −1 and CCCP (50 µM) as positive control. The results are presented as relative fluorescence units (RFU) and given as means ± SD ( n = 6). & p < 0.5, # p < 0.01, * p < 0.001 vs. untreated control.

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques: Membrane, Positive Control, Fluorescence, Control

Journal: Toxics

Article Title: PBDEs Found in House Dust Impact Human Lung Epithelial Cell Homeostasis

doi: 10.3390/toxics10020097

Figure Lengend Snippet: Percentage of different stages of apoptosis of A549 cells after 4 h exposure to single PBDE congener and house dust sample extract at 12 µg mL −1 and PFA (0.08%, v / v ) as a positive control. The results are presented as percentage of total apoptotic cells compared to vehicle control (2.4% methanol) and given as means ± SD ( n = 6). * p < 0.001 vs. control.

Article Snippet: The certified human carcinoma alveolar basal epithelial A549 cell line (ECACC 86012804) was purchased from the European Collection of Authenticated Cell Cultures (ECACC) cell bank.

Techniques: Positive Control, Control